ON PHOSPHOTUNGSTIC ACID STAINING. II

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The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid

After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...

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The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid Lloyd

After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...

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Selective Demonstration of a Type of Synaptic Vesicle by Phosphotungstic Acid Staining

Fine structural analyses of nerve terminals in both the central and in the peripheral nervous systems have demonstrated the presence of synaptic vesicles, which were postulated to be the storage sites of neurotransmitters . Several different types of synaptic vesicles have been described (see ref . 6) . Electron-lucent vesices, 400-500 A in diameter, constitute the most commonly occurring type ...

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Photocatalytic Decolorization of Methyl Orange Solution with Phosphotungstic Acid

Photocatalytic decolorization of Methyl Orange (MO) solution has been studied using phosphotungstic acid (HPA) as photocatalyst. The decolorization reaction of MO solution is photocatalysis. The paper reveals that the optimal loading of photocatalyst for decolorization of MO solution (10 mg/L) is 0.6 g/L. The results show that the photocatalytic decolorization reaction of MO with HPA in a h...

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Interference of the chemotherapeutic agent etoposide with the direct phosphotungstic acid method for uric acid.

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ژورنال

عنوان ژورنال: Journal of Histochemistry & Cytochemistry

سال: 1971

ISSN: 0022-1554,1551-5044

DOI: 10.1177/19.11.648